AUXIN-KINETIN INTERACTION REGULATING THE SCOPOLETIN AND SCOPOLIN LEVELS IN TOBACCO TISSUE CULTURES

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Abstract
Tobacco tissue cultured in vitro releases into the medium a fluorescent material the main portion of which has been identified as scopoletin. The amount of scopoletin released varies with the concentration of auxin in the medium and increases markedly with high, toxic levels of auxin. It has been shown that the scopoletin derives from a glycoside (scopolin). Kinetin added to the medium prevents the release of scopoletin by indoleacetic acid (IAA) and permits the maintenance of high glucoside levels even in the presence of high, nontoxic levels of IAA. With toxic IAA levels, treatment with kinetin becomes ineffective. It is suggested that the auxin-kinetin levels regulate the scopoletin-scopoletin glycoside equilibria in the tissues and the conversion of these substances into cell-wall materials. This process is considered as a part of the system in which auxin and kinetin interact in regulating growth and organ formation in plant tissues.