Assignment of human 3-hydroxy-3-methylglutaryl coenzyme A reductase gene to q13 → q23 region of chromosome 5
- 1 January 1986
- journal article
- research article
- Published by Springer Nature in Somatic Cell and Molecular Genetics
- Vol. 12 (1) , 89-94
- https://doi.org/10.1007/bf01560731
Abstract
We have used hamster cDNA probes for 3-hydroxy-3-methylglutaryl coenzyme A (HMG CoA) reductase (HMGCR) to determine the chromosomal location of the human gene for HMG CoA reductase. Southern blot analysis of genomic DNA from 16 independent mouse-human somatic cell hybrids showed that the human gene for HMG CoA reductase resides on chromosome 5. Analysis of Chinese hamster-human somatic cell hybrids selectively retaining human 5 or a portion of it showed that the gene locus for HMG CoA reductase can be assigned to the q13 → q23 region of chromosome 5.Keywords
This publication has 21 references indexed in Scilit:
- Detection of specific sequences among DNA fragments separated by gel electrophoresisPublished by Elsevier ,2006
- Molecular approach to analyzing the human 5p deletion syndrome, cri du chatSomatic Cell and Molecular Genetics, 1985
- HMG CoA reductase: A negatively regulated gene with unusual promoter and 5′ untranslated regionsCell, 1984
- Nucleotide sequence of 3-hydroxy-3-methyl-glutaryl coenzyme A reductase, a glycoprotein of endoplasmic reticulumNature, 1984
- A technique for radiolabeling DNA restriction endonuclease fragments to high specific activityAnalytical Biochemistry, 1983
- The transfer and stable integration of the HSV thymidine kinase gene into mouse cellsCell, 1978
- Labeling deoxyribonucleic acid to high specific activity in vitro by nick translation with DNA polymerase IJournal of Molecular Biology, 1977
- Ouabain as a selective agent in the isolation of somatic cell hybridsCytogenetic and Genome Research, 1975
- Selection of Hybrids from Matings of Fibroblasts in vitro and Their Presumed RecombinantsScience, 1964
- GENETICS OF HUMAN CELL LINES, IV. DNA-MEDIATED HERITABLE TRANSFORMATION OF A BIOCHEMICAL TRAITProceedings of the National Academy of Sciences, 1962