Induction of protooncogene fos by extracellular signals in primary glial cell cultures

Abstract
In the present study various extracellular factors, acting through different second messenger systems, were examined for their capacity to increase the level of c‐fos mRNA in primary glial cell cultures. In particular EGF, 12‐O‐tetradecanoylphorbol 13‐acetate, the beta‐adrenergic agonist isoproterenol, and the glutamate agonists, ibotenic and quisqualic acid, were studied. All the extracellular stimuli tested induced a rapid and transient increase in c‐fos mRNA level in glial cell cultures regardless of the signal transduction pathway and the final effect on cell proliferation.