Microbiological Estimation of Antifungal Blood Levels Following Administration of Fungicides

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Abstract
Applying as a standard the growth of Candida tropicalis (Castellani) Berkhout in 50 percent normal guinea pig serum containing dilutions of fungicide, the antifungal activity of guinea pig serum obtained after intraperitoneal injection of various antifungal drugs was measured. Results were reproducible within satisfactory limits and recovery from the test sera of added known amounts of fungicide was good. While no in vivo levels were detectable for actidione, o-dichloroacetyloxine, prodigiosin, or stilbamidine isethionate under the imposed experimental conditions (dose of 16 mgm per kg regardless of biological potency of drug; blood taken approximately three hours post injection), substantial serum concentrations ranging from 1.2 to 28 meg per ml were found for ascosin, candicidin, 3-keto-21-piperidyl)-Δ4-pregnadiene monohydrobro-mide, nystatin, and rimocidin. It is proposed that further development of such microbiological technique may furnish a valuable tool in the laboratory investigation and evaluation of antifungal drugs for therapeutic use.