SELECTIVE PHAGOCYTOSIS OF CRYSTALLINE METAL SULFIDE PARTICLES AND DNA STRAND BREAKS AS A MECHANISM FOR THE INDUCTION OF CELLULAR-TRANSFORMATION

  • 1 January 1982
    • journal article
    • research article
    • Vol. 42  (7) , 2757-2763
Abstract
Crystalline NiS, CuS, CoS2 and CdS particles were actively phagocytosed by cells and potently induced morphological transformation of Syrian hamster embryo cells in a concentration-dependent fashion. In contrast, the respective amorphous metal sulfide particles (amorpous NiS, CuS, CoS, and CdS) were not as actively phagocytosed by cultured cells and, in comparison to the crystalline form of these compounds, induced considerbly less morphological transformation at both cytotoxic and noncytotoxic exposure levels. Chemical reduction of positively charged amorphous NiS with LiAIH4 resulted in active phagocytosis of these particles which was also associated with enhancement of cellular transformation. Crystalline but not amorphous NiS caused considerable strand breaks in the DNA of Chinese hamster ovary cells following 2-3 h exposure at 10 .mu.g/ml as determined by alkaline sucrose gradient techniques with subsequent determination of DNA molecular weight. Phagocytized inert particles such as latex beads did not induce transformation or DNA damage, suggesting that genotoxic dissolution products such as Ni2+ rather than the phagocytized particles are responsible for the observed DNA damage and cellular transformation. NiCl2 was .apprx. 1/3-1/2 as potent in inducing cellular transformation compared to crystalline NiS on a weight basis. These results correlate the selective phagocytosis of crystalline metal sulfides to their more potent activity in the induction of cellular transformation.