Mitochondrial preparations from the leaves of tobacco (Nicotiana tabacum). 4. Separation of some components by density-gradient centrifuging

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Abstract
Preparations from tobacco leaves were centrifuged in sucrose density gradients and resolved into chloroplasts, chloroplast fragments and particles, presumably mitochondria, containing succinoxidase activity. The centrifuging sedimented fumarase as if it were attached to the mitochondria. Cytochrome oxidase sedimented as if it were associated with mitochondria, and some mitochondrial fragments. No activity was detected in chloroplasts either before or after disruption with digitonin. Glycollic oxidase did not sediment appreciably during the centrifuging. It could not be detected in intact chloroplasts.