CELL-MEDIATED CYTOTOXICITY OF LYMPHOCYTES FROM CHICKENS INOCULATED WITH HERPESVIRUS OF TURKEY AGAINST A MAREKS-DISEASE LYMPHOMA CELL-LINE (MSB-1)

  • 1 January 1979
    • journal article
    • research article
    • Vol. 22  (1) , 11-20
Abstract
Using a new device which increases the sensitivity of detection of specific immune lysis of target cells by labeling them with [35S]-methionine, the in vitro cell-mediated cytotoxic response of spleen lymphocytes and peripheral blood lymphocytes from chickens vaccinated with herpesvirus of turkey (HVT), O1 strain, against MSB-1 line cells was clearly demonstrated. The cytotoxic activity was clearly inhibited by pre-treatment of effector lymphocytes with anti-T [thymus-derived] lymphocyte serum and complement. The activity was greater using T cells purified from spleen lymphocytes and peripheral blood lymphocytes than with the unfractionated cells, indicating that T lymphocytes play the main role in effector activity. Using sera from HVT-vaccinated chickens, no significant cytotoxic effects were detected in the complement-dependent antibody cytotoxicity test against MSB-1 cells. Cellular immunity against the surface antigen of Marek''s disease (MD) lymphoma cells may be mainly related to the preventive mechanism against MD incidence by HVT vaccination.

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