Methionine biosynthesis in Saccharomyces cerevisiae

Abstract
In order to analyse how many structural genes are implicated in the specifi steps of the biosynthesis of methionine in Sacch. cerevisiae, a hundred mutants were studied by complementation. 21 groups were defined named MET1 to MET25. Neither recombination between independent mutants of the same complementation group nor linkage between different groups was found. Preliminary to biochemical studies, mutants of each complementation group were tested for their capacity to utilize various precursors of methionine.