PULMONARY CLEARANCE OF HEMOPHILUS-PLEUROPNEUMONIAE AND SERRATIA-MARCESCENS IN MICE

  • 1 January 1982
    • journal article
    • research article
    • Vol. 43  (10) , 1799-1801
Abstract
Bacterial counts (per lung) were made from Swiss White and/or C3H mice, inoculated intranasally with either H. pleuropneumoniae or S. marcescens and killed at specific times. The percentages of bacterial survival and clearance at each time were determined. S. marcescens was cleared progressively, but not completely, from the lungs of C3H and Swiss White mice. The overall pulmonary clearance of S. marcescens by Swiss White mice was significantly greater than that of C3H mice (P < 0.01). The pulmonary clearance pattern of H. pleuropneumoniae in C3H mice differed according to the dose inoculated. With a larger H. pleuropneumoniae LD50 (0.1 LD50), the organism multiplied consistently up to 25 times by 12 h after the inoculations were done, when clinical signs and lesions appeared in the few of the mice. The clearance rates at 24 and 48 h after inoculations were 60.65 and 10.3%, respectively. Mice given 0.04 LD50 H. pleuropneumoniae showed a 10-fold increase of H. pleuropneumoniae in the first 4 h, with clearances reaching 33, 66, and 99% at 8, 12 and 24 h, respectively.