Effect of ethanol on hepatic metabolism of sulfobromophthalein

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Abstract
Hepatic uptake, storage, conjugation, and excretion of sulfobromophthalein (BSP) (2 mg/100 g body wt, administered iv) were measured in rats. Conjugated BSP in bile or extract of liver was identified by a method using paper chromatography. Ethanol, administered intraperitoneally in a dose of 120 mg/100 g body wt, caused a decrease in the proportion of conjugated BSP in storage and in the rate of excretion of conjugated BSP in bile, but did not affect the rate of uptake, excretion of unconjugated BSP, or the ability of the liver to excrete administered BSP-glutathione mercaptide. Ethanol decreases the rate of excretion of BSP by impairing conjugation with glutathione. Hepatic cellular mechanisms for uptake of dye from plasma and transfer of dye from cell to bile are independent of the mechanism for conjugation and conjugation is a rate-determining step in excretion of BSP.