AUTORADIOGRAPHIC LOCALIZATION OF OESTROGEN IN RAT TISSUES

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Abstract
SUMMARY: The intracellular localization of [6,7-3H]oestradiol in rat pituitary, liver and uterus has been studied using autoradiography. In the anterior pituitary the oestrogen has been located on the nuclear membrane and nucleolus of the eosinophils. The posterior pituitary did not produce positive autoradiographs and only occasional silver grains were seen in the pars intermedia. In the liver the silver grains were uniformly distributed throughout the cytoplasm; only very occasionally were grains associated with the nucleus. No satisfactory results were obtained with the uterus. Labelled erythrocytes were seen in all the tissues studied. The results are discussed in relation to the mode of action of oestradiol.