Identification and quantitative analysis of degradation products of chlorhexidine with chlorhexidine‐resistant bacteria with three‐dimensional high performance liquid chromatography

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Abstract
The products of the degradation of chlorhexidine by two chlorhexidine‐resistant strains of Achromobacter xylosoxidans, isolated from an ultrasonic hand washer, were identified by three‐dimensional HPLC. In the degradation process a pathway forming p‐chlorophenol, probably through p‐chloroaniline, was also observed, as was a pathway forming phenol and pyrogallol. The quantitative measurement of these products at their maximum absorption spectra by two‐dimensional HPLC suggested the presence of chlorhexidine‐degrading enzymes.