Sequential observations on the appearance of neoplastic lesions in the liver and kidney after treatment with N-ethyl-N-hydroxyethylnitros-amine followed by partial hepatectomy and unilateral nephrectomy
- 1 January 1983
- journal article
- research article
- Published by Oxford University Press (OUP) in Carcinogenesis: Integrative Cancer Research
- Vol. 4 (5) , 523-528
- https://doi.org/10.1093/carcin/4.5.523
Abstract
Sequential observations were carried out on the induction of preneoplastic lesions in the liver and the kidney. Rats were initially given N-ethyl-N-hydroxyethylnitrosamine (EHEN) in their drinking water (0.1%) for 3 days (Group 1), 1 week (Group 2) or 2 weeks (Group 3) or tap water (Group 4). Rats in Groups 1 – 3 were subjected to partial hepatectomy and unilateral nephrectomy (right side) 2 weeks after the end of EHEN treatment. Rats from these groups were killed in week 10, 20, 30 and 40 of the experiment. In the liver, the effect of EHEN in the induction of γ-glutamyltranspeptidase (γ-GT) positive foci and hyperplastic nodules (HN) was clearly dependent on the length of treatment. The preneoplastic lesions increased with the lapse of observation time. Changes measured as number of γ-GT positive foci were 10–40 times greater than those measured as HN, especially among the small size range. Values for changes in Group 1 given 0.1% EHEN for 3 days were very low, indicating that this dose is close to the threshold. Two rats with hepatocellular carcinoma in Group 3 given EHEN for 2 weeks survived until week 40. In the kidney, tubular epithelial proliferations composed of cells with slightly basophilic cytoplasm and slightly atypical nuclei were tentatively named atypical cell foci (ACF). EHEN induced ACF, renal cell adenomas and renal cell carcinomas. The increase in the induction of ACF was dependent on the length of observation period but not on the length of treatment. Even though control rats (not treated with EHEN) also had ACF, their quantitative values were far less than the groups given EHEN and killed at week 40, indicating that a large number of ACF were induced by EHEN. Therefore, EHEN is good for experimental induction of preneoplastic lesions in the liver and kidney of rats. The experimental schedule for Groups 1 and 2 could be used as a short-term screening test for promoters and the schedule for Group 3 as an assay for inhibitors.Keywords
This publication has 11 references indexed in Scilit:
- INDUCTION OF GAMMA-GLUTAMYL-TRANSFERASE TRANSPEPTIDASE POSITIVE FOCI IN RAT-LIVER BY PYROLYSIS PRODUCTS OF AMINO-ACIDS1981
- Adenocarcinoma of The Kidney. III. Histogenesis of Renal Adenocarcinomas Induced in Rats by N-(4′-Fluoro-4-biphenylyl)acetamide234JNCI Journal of the National Cancer Institute, 1980
- PHENOTYPIC DIVERSITY AS AN EARLY PROPERTY OF PUTATIVE PRENEOPLASTIC HEPATOCYTE POPULATIONS IN LIVER CARCINOGENESIS1980
- CARCINOGENICITY OF N-NITROSOMETHYL(2-OXOPROPYL)AMINE IN SYRIAN-HAMSTERS1980
- INDUCTION OF RESISTANT HEPATOCYTES AS A NEW PRINCIPLE FOR A POSSIBLE SHORT-TERM INVIVO TEST FOR CARCINOGENS1980
- HISTOPATHOLOGICAL STUDIES ON RENAL TUBULAR CELL TUMORS IN RATS TREATED WITH N-ETHYL-N-HYDROXYETHYLNITROSAMINE1979
- SEQUENTIAL-ANALYSIS OF HEPATIC CARCINOGENESIS - COMPARATIVE-STUDY OF THE ULTRASTRUCTURE OF PRENEOPLASTIC, MALIGNANT, PRENATAL, POSTNATAL, AND REGENERATING LIVER1979
- Carcinogenic Effects of Diisopropanolnitrosamine in Sprague-Dawley RatsJNCI Journal of the National Cancer Institute, 1977
- HISTOCHEMICAL DEMONSTRATION OF INCREASED ACTIVITY OF γ-GLUTAMYL TRANSPEPTIDASE IN RAT LIVER DURING HEPATOCARCINOGENESISACTA HISTOCHEMICA ET CYTOCHEMICA, 1976
- Induction of kidney tumours in the rat with dimethylnitrosamine (N‐nitrosodimethylamine)The Journal of Pathology and Bacteriology, 1962