Aminoacy1 Transfer RNA Formation. VI. Binding of Cations to Transfer RNA and Its Role in Aminoacy1 Transfer RNA Formation1
- 1 September 1976
- journal article
- research article
- Published by Oxford University Press (OUP) in The Journal of Biochemistry
- Vol. 80 (3) , 463-469
- https://doi.org/10.1093/oxfordjournals.jbchem.a131299
Abstract
The role of cations (polyamines and Mg2+) in isoleucyl-tRNA formation catalyzed by purified isoleucyl-tRNA synthetase [EC 6.1.1.5] from Escherichia coli was studied. It was found that spermine, spermidine, and Mg2+ bind to tRNA and that when bound to these cations, tRNA acts as substrate of aminoacylation without requiring further cations. These findings suggest that the primary function of cations in aminoacyl-tRNA formation is to bind to tRNA to stablize its structure, not to bind to the enzyme to activate it.Keywords
This publication has 2 references indexed in Scilit:
- ENZYMATIC CARBOXYL ACTIVATION OF AMINO ACIDSPublished by Elsevier ,2021
- MECHANISM OF AMINOACYL RNA SYNTHESIS: STUDIES WITH ISOLATED AMINOACYL ADENYLATE COMPLEXES OF ISOLEUCYL RNA SYNTHETASEProceedings of the National Academy of Sciences, 1964