Activation of the dephosphorylation of nitrogen regulator I-phosphate of Escherichia coli
- 1 February 1995
- journal article
- Published by American Society for Microbiology in Journal of Bacteriology
- Vol. 177 (4) , 926-931
- https://doi.org/10.1128/jb.177.4.926-931.1995
Abstract
The transcription of sigma 54 RNA polymerase-dependent nitrogen-regulated genes is activated by nitrogen regulator I (NRI)-phosphate. The kinase NRII is responsible for the phosphorylation of NRI. It has been shown that NRII also has the ability to dephosphorylate NRI-phosphate but only when PII is present at a concentration greatly in excess of that of NRII. We have now shown that glutamate enables PII to stimulate the dephosphorylation of NRI-phosphate when present in equimolar concentration with NRII. This effect of glutamate appears to be a backup control that becomes effective when the normal regulation of PII activity is disabled.Keywords
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