A comparison of the sensitivity of immunofluorescence and Giemsa for staining Chlamydia trachomatis inclusions in cycloheximide-treated McCoy cells.

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Abstract
In a clinical study of 190 men with non-gonococcal urethritis, C. trachomatis inclusions were sought in cycloheximide-treated McCoy [human synovial] cells by an indirect immunofluorescent antibody technique. The method was consistently reliable for 2 yr and the results were obtained within 24 h of a patient''s attendance. The results correlated with those obtained by Giemsa staining in 91.6% of patients and the new method was at least as sensitive as the established Giemsa-staining method.