Cultivation of Chlamydia trachomatis in cycloheximide-treated mccoy cells

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Abstract
An isolation technique for C. trachomatis using McCoy [human synovial] cells is described. In contrast to earlier techniques employing such cells, no pretreatment of cells was used. Glutarimide antibiotic cycloheximide was added to the culture medium used for incubating the cells after infection. Cycloheximide was used at concentrations that depressed, but did not completely inhibit metabolism of the eukaryotic host cells. In studies on different immunotypes of C. trachomatis cultured in the yolk sac of embryonated hen eggs, the cycloheximide technique was compared with a method using pretreatment of cells with 5-iodo-2-deoxyuridine. The cycloheximide method gave greater numbers of inclusion-forming units per cover slip for all the immunotypes of trachoma-inclusion conjunctivitis agents tested, i.e., A-I. In a study of 194 cervical and urethral specimens from women, cycloheximide treatment of McCoy cells apparently was more efficient than 5-iodo-2-deoxyuridine treatment for isolation of C. trachomatis.