Identification of carcinogenic tryptophan pyrolysis products in human bile by high‐performance liquid chromatography
- 1 January 1990
- journal article
- research article
- Published by Wiley in Environmental and Molecular Mutagenesis
- Vol. 15 (4) , 229-235
- https://doi.org/10.1002/em.2850150410
Abstract
The carcinogenic tryptophan pyrolysis products 3‐amino‐1,4‐dimethyl‐5H‐pyrido[4,3‐b]indole (Trp‐P‐1) and 3‐amino‐1‐methyl‐5H‐pyrido[4,3‐b]indole (Trp‐P‐2) have been demonstrated to be present in human bile through use of a highperformance liquid chromatography (HPLC) method. The method consists of the acid‐induced release of the carcinogens from bile components and their extraction with methylene chloride and subsequent quantification by HPLC. In seven subjects who had received cath‐eterization of the bile duct and external biliaryd‐rainage, the average amounts of Trp‐P‐1 and Trp‐P‐2 excreted daily in the bile were 408 fmol/ day (n = 7) and 864 fmol/day (n = 7), respectively. In one subject, furthermore, significant daily changes of these carcinogen levels in bile and plasma were confirmed during 2 weeks of observation. These results indicate that one of the excretory pathways of these carcinogens is via bile. Our data also may suggest that Trp‐P‐1 and Trp‐P‐2 are derived from everyday foods.Keywords
This publication has 22 references indexed in Scilit:
- Carcinogenic tryptophan pyrolysis products in airborne particles and rain waterMutation Research Letters, 1989
- Metabolic Activation of Pyrolysate Arylamines by Human Liver Microsomes; Possible Involvement of a P-448-H Type Cytochrome P-450Japanese Journal of Cancer Research, 1988
- Analysis of human plasma as an exposure level monitor for carcinogenic tryptophan pyrolysis productsMutation Research Letters, 1988
- In vitro and in vivo N-acetylation of carcinogenic glutamic acid pyrolysis products in humansCarcinogenesis: Integrative Cancer Research, 1988
- Detection of Trp-P-1 and Trp-P-2, carcinogenic tryptophan pyrolysis products, in dialysis fluid of patients with uremiaMutation Research - Fundamental and Molecular Mechanisms of Mutagenesis, 1987
- Activation of mutagens in cooked ground beef by human-liver microsomesMutation Research Letters, 1984
- Urinary mutagens in humans after fried pork and bacon mealsCancer Letters, 1984
- Chromosome Aberrations and Sister Chromatid Exchanges Induced by Tryptophan Pyrolysates, Trp-P-1 and Trp-P-2, in Cultured Human and Chinese Hamster CellsProceedings of the Japan Academy, Series B, 1980
- Malignant transformation in vitro by tryptophan pyrolysis products.Proceedings of the Japan Academy, Series B, 1978