The phosphatidylinositol kinase of rat brain
- 1 November 1966
- journal article
- research article
- Published by Portland Press Ltd. in Biochemical Journal
- Vol. 101 (2) , 328-337
- https://doi.org/10.1042/bj1010328
Abstract
The presence of a phosphatidylinositol kinase in homogenates of adult rat brain was shown by using labeled ATP [adenosine triphosphate] or labeled phosphatidylinositol. The kinase was activated by Mg2+ or Mn2+ and inhibited by Ca2+, Cu2+, K+, Na+ and F-. The detergents sodium deoxycholate, Cutscum and Triton X-100 markedly stimulated the reaction; sodium taurocholate, Tween-20 and cetyltrimethylammonium bromide were less effective. The activity of the enzyme was dependent on SH groups. The subcellular distribution of the kinase in brain resembled that of Na+-plus-K+-stimulated adenosine triphosphatase and 5[image] -nucleotidase.This publication has 19 references indexed in Scilit:
- Preparation and properties of various salt forms of plant phosphatidyl inositolsLipids, 1966
- Incorporation of phosphate into diphosphoinositide by subcellular fractions from liverBiochimica et Biophysica Acta (BBA) - Lipids and Lipid Metabolism, 1965
- Studies on the fractionation of mucosal homogenates from the small intestineBiochemical Journal, 1965
- The site of diphosphoinositide synthesis in rat liverBiochemical and Biophysical Research Communications, 1965
- Biosynthesis of Diphosphoinositide in BrainJournal of Biological Chemistry, 1965
- The incorporation of 32P from triphosphate into polyphosphoinositides [γ-32P]adenosine and phosphatidic acid in erythrocyte membranesBiochimica et Biophysica Acta (BBA) - Specialized Section on Lipids and Related Subjects, 1964
- Ion Exchange Chromatography of Intact Brain Phosphoinositides on Diethylaminoethyl Cellulose by Gradient Salt Elution in a Mixed Solvent SystemJournal of Biological Chemistry, 1964
- A simple method for the preparation of 32P-labelled adenosine triphosphate of high specific activityBiochemical Journal, 1964
- The distribution of adenosine triphosphate in subcellular fractions of brain tissueBiochemical Journal, 1963
- Further studies on the properties and assay of glucose 6-phosphate dehydrogenase and 6-phosphogluconate dehydrogenase of rat liverBiochemical Journal, 1953