Fluorescence labeling of the C‐terminus of proteins with a puromycin analogue in cell‐free translation systems
Open Access
- 22 November 1999
- journal article
- Published by Wiley in FEBS Letters
- Vol. 462 (1-2) , 43-46
- https://doi.org/10.1016/s0014-5793(99)01474-x
Abstract
We have developed a new method for the C‐terminus‐specific fluorescence labeling of proteins. This method is based on the experimental finding that a fluorescent puromycin analogue at lower concentra...Keywords
This publication has 16 references indexed in Scilit:
- Minithioredoxin: A Folded and Functional Peptide Fragment of ThioredoxinBiochemical and Biophysical Research Communications, 1999
- In vitro Trans Translation mediated by alanine-charged 10sa RNAJournal of Molecular Biology, 1997
- Role of a Peptide Tagging System in Degradation of Proteins Synthesized from Damaged Messenger RNAScience, 1996
- Fluorescence polarization — a new tool for cell and molecular biologyNature, 1995
- A bifunctional fusion protein containing Fc-binding fragment B of staphylococcal protein A amino-terminal to antidigoxin single-chain FvBiochemistry, 1990
- tRNA‐mediated labelling of proteins with biotinEuropean Journal of Biochemistry, 1988
- Polymer support oligonucleotide synthesis XVIII1.2): use ofβ-cyanoethyi-N,N-dialkylamino-/N-morpholino phosphoramidite of deoxynucleosides for the synthesis of DNA fragments simplifying deprotection and isolation of the final productNucleic Acids Research, 1984
- Repetitive Sequences in Protein A from Staphylococcus aureusEuropean Journal of Biochemistry, 1977
- PUROMYCIN INHIBITION OF PROTEIN SYNTHESIS: INCORPORATION OF PUROMYCIN INTO PEPTIDE CHAINSProceedings of the National Academy of Sciences, 1964
- Structural Requirements for Puromycin Inhibition of Protein SynthesisNature, 1963