Influence of hyperthermia on the phosphorylation of ribosomal protein S6 from human skin fibroblasts and meningioma cells
- 21 March 1983
- journal article
- Published by Wiley in FEBS Letters
- Vol. 153 (2) , 262-266
- https://doi.org/10.1016/0014-5793(83)80620-6
Abstract
Skin fibroblasts and meningioma cells, derived from primary cultures of the same patients have been used to study the influence of hyperthermia on (i) cell morphology and (ii) phosphorylation pattern of ribosomal and ribosome-associated proteins. Incubation of tumour cells and fibroblasts up to 7 h at 42°C did not significantly change the cell morphology as compared to control cells kept at 37°C. At 42°C ribosomal protein S6 is shifted cathodically indicating a loss of negative charge, however no quantitative dephosphorylation of S6 was observed. Meningioma cells and fibroblasts did not differ significantly with respect to S6 phosphorylation.Keywords
This publication has 18 references indexed in Scilit:
- Regulation of the Aminoacyl‐tRNA Synthetase Complex of Rat Liver by Phosphorylation/Dephosphorylation in vitro and in vivoEuropean Journal of Biochemistry, 1982
- Heat-shock-induced alterations of ribosomal protein phosphorylation in plant cell culturesCell, 1982
- HeLa cell messenger RNAs of widely differing sizes encode certain heat shock proteins in theMrregion of 72 000-74 000FEBS Letters, 1982
- Modulation of heat shock polypeptide synthesis in HeLa cells during hyperthermia and recoveryBiochemistry, 1982
- Rapid and efficient method for analyzing phosphorylation of the S6 ribosomal protein in 32Pi-labeled, tissue culture cellsAnalytical Biochemistry, 1981
- Characterization of native 40 S particles from Krebs II mouse ascites tumor cells. Resolution, nomenclature and molecular weights of the nonribosomal proteinsBiochimica et Biophysica Acta (BBA) - Nucleic Acids and Protein Synthesis, 1981
- Insulin‐like growth factor: insulin or serum increase phosphorylation of ribosomal protein S6 during transition of stationary chick embryo fibroblasts into early G1 phase of the cell cycleFEBS Letters, 1979
- Phosphorylation of ribosomal protein S6 in suspension cultured HeLa cellsMolecular Genetics and Genomics, 1977
- Regulation of protein synthesis in HeLa cells: Translation at elevated temperaturesJournal of Molecular Biology, 1969