ExoT of Cytotoxic Pseudomonas aeruginosa Prevents Uptake by Corneal Epithelial Cells
Open Access
- 1 January 2000
- journal article
- Published by American Society for Microbiology in Infection and Immunity
- Vol. 68 (1) , 403-406
- https://doi.org/10.1128/iai.68.1.403-406.2000
Abstract
The presence of invasion-inhibitory activity that is regulated by the transcriptional activator ExsA of cytotoxic Pseudomonas aeruginosa has previously been proposed. The results of this study show that both ExoT and ExoS, known type III secreted effector proteins of P. aeruginosa that are regulated by ExsA, possess this activity. Invasion was reduced 94.4% by ExoT and 96.0% by ExoS. Invasion-inhibitory activity is not linked to ADP-ribosylation activity, at least for ExoS, since a noncatalytic mutant also inhibits uptake by an epithelial cell line (invasion was reduced 96.0% by ExoSE381A).Keywords
This publication has 37 references indexed in Scilit:
- Biological effects of Pseudomonas aeruginosa type III-secreted proteins on CHO cells.1999
- Use of an Animal Model in Studies of Bacterial Corneal InfectionILAR Journal, 1999
- ExoY, an adenylate cyclase secreted by thePseudomonas aeruginosatype III systemProceedings of the National Academy of Sciences, 1998
- Identification of type III secreted products of the Pseudomonas aeruginosa exoenzyme S regulonJournal of Bacteriology, 1997
- Gapped BLAST and PSI-BLAST: a new generation of protein database search programsNucleic Acids Research, 1997
- ExoU expression by Pseudomonas aeruginosa correlates with acute cytotoxicity and epithelial injuryMolecular Microbiology, 1997
- YopH of Yersinia pseudotuberculosis interrupts early phosphotyrosine signalling associated with phagocytosisMolecular Microbiology, 1996
- Protein Tyrosine Phosphatase Activity of an Essential Virulence Determinant in YersiniaScience, 1990
- The cytotoxic protein YopE of Yersinia obstructs the primary host defenceMolecular Microbiology, 1990
- The plasmid‐encoded Yop2b protein of Yersinia pseudotuberculosis is a virulence determinant regulated by calcium and temperature at the level of transcriptionMolecular Microbiology, 1988