Expression of cloned cDNA for a major surface antigen of Plasmodium falciparum merozoites
- 23 July 1984
- journal article
- Published by Wiley in FEBS Letters
- Vol. 173 (1) , 108-112
- https://doi.org/10.1016/0014-5793(84)81027-3
Abstract
A cDNA library of P. falciparum was constructed. Using size-selected mRNA as a probe several clones were isolated which hybridized to mRNAs larger than 5 kilobases (kb). The cDNA insert of pFC 17, which hybridizes to 5.6-kb mRNA was expressed by fusion to anthranilate synthetase I in a plasmid expression vector. The expressed fusion protein was shown to contain epitopes of a 195-kDa protein which is the precursor to 3 major surface antigens of P. falciparum merozoites.Keywords
This publication has 12 references indexed in Scilit:
- Cloning of malarial genes coding for high molecular weight antigens: Isolation of fragments of Plasmodium falciparum genes coding for proteins of 145 000 molecular weightMolecular and Biochemical Parasitology, 1984
- Serological cross-reaction between high molecular weight proteins synthesized in blood schizonts of Plasmodium yoelii, Plasmodium chabaudi and Plasmodium falciparumMolecular and Biochemical Parasitology, 1983
- Surface antigens of malaria merozoites. A high molecular weight precursor is processed to an 83,000 mol wt form expressed on the surface of Plasmodium falciparum merozoites.The Journal of Experimental Medicine, 1983
- Biosynthesis and processing of a Plasmodium falciparum schizont antigen recognized by immune serum and a monoclonal antibody.The Journal of Experimental Medicine, 1982
- The pUC plasmids, an M13mp7-derived system for insertion mutagenesis and sequencing with synthetic universal primersGene, 1982
- TECHNOLOGICAL EXAMINATION OF LOW‐FIRED TERRACOTTA STATUES FROM AYIA IRINI, KEAArchaeometry, 1982
- Antigenic Diversity in the Human Malaria Parasite Plasmodium falciparumScience, 1982
- Immunization against blood-stage rodent malaria using purified parasite antigensNature, 1981
- Trans-complementable copy-number mutants of plasmid ColE1Nature, 1980
- Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4Nature, 1970