Subcellular Distribution of Ubiquinone and the Content of Ubiquinone and Other Electron Carriers in the Mitochondria Isolated from Tobacco Cultured Cells

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Abstract
The intracellular distribution of UQ in BY-2 cells was examined. The distribution of UQ is well correlated with the distribution of Cyt c oxidase activity in mitochondria. Mitochondrial preparations from BY-2 cells examined showed a high degree of both coupling and respiratory control. They were further purified by sucrose density gradient centrifugation and UQ and Cyts contents in the purified mitochondria were determined. The UQ and Cyts (b and c) contents and their molar ratios in BY-2 cell mitochondria are similar to those of mung bean seedling mitochondria.