The Use of Synthetic Medium As an in Vitro Test of Possible Chemotherapeutic Agents Against Gram-Negative Bacteria

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Abstract
Summary and Conclusions: A readily reproducible synthetic medium which supported growth of Vibrio cholera, Pasteurella multocida, Shigella dysenteriae, Eberthella typhosa, and Salmonella pullorum was used over a period of two and a half years in in vitro tests of the activity of more than 1000 compounds. Approximately 15 per cent of these chemicals were able to inhibit completely the growth in this medium. Chemicals whose in vitro activity was also demonstrated in vivo were all recently developed sulfonamides such as 2-sulfanilamidopyrimidine, 2-sulfanilamidopyrazine, 2-sulfanilamidopyridine and their heterocyclic substituted chloro-, bromo-, and methyl compounds. None of the compounds showing in vivo activity as demonstrated by Little et al. (in press) failed to show in vitro activity.