A Specific Method for the Measurement of Tacrolimus in Human Whole Blood by Liquid Chromatography/Tandem Mass Spectrometry

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Abstract
Tacrolimus (FK 506) measurement by immunoassays in clinical samples of organ transplant patients often lacks a specific reference method. A method combining liquid chromatography (LC) with tandem mass spectrometry (MS/MS) was developed to quantify tacrolimus in whole blood. Liquid-liquid extraction was performed on 1 ml of sample before narrow-bore LC/MS/MS analysis. Ascomycin was used as an internal standard. The standard curve was composed of seven points ranging from 1 to 50 µg/l (average r2 = 0.9999). Limits of detection and quantitation were 0.25 and 0.75 µg/l, respectively. Imprecision was y

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