Effects of Solvent Upon Absorption Spectra of Chlorophylls A and B; Their Ultraviolet Absorption Spectra in Ether Solution

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Abstract
Solns. of chlorophyll a were prepared in 13 solvents and of component b in 5 solvents by direct elution from sucrose adsorption columns. Several ethers, alcohols, hydrocarbons, CCl4, methyl oleate, and olive oil were employed. Spectro-photometric curves for the visible region were plotted on a relative basis and compared with the standard curve for ethyl ether solns. Spectra for the u.-v. region to 2650 A were detd. for solns. in ethyl ether. A prepn. of dried chlorophyll [alpha] is reported which retained excellent spectro-scopic properties following drying. Kundt''s Rule is discussed in relation to the chem. nature of the solvent. It is shown that this rule cannot be applied to chlorophyll solns. in general but the limited data are favorable to Kundt''s rule only when applied to solvents of similar chemical nature. The relation of the spectral characteristics to analytical applications and choice of solvent is discussed. In general, spectra of the alcoholic solns. do not have the minor bands well resolved. Spectra of methanolic solns. are particularly deficient in structural detail.