THE FLUORESCENCE OF EIGHT NATURAL ESTROGENS IN SULPHURIC ACID

Abstract
The sulphuric acid fluorescence and absorption spectra of eight natural estrogens (estrone, estradiol-17β, estriol, 16-epiestriol, 16-ketoestradiol-17β, 16α-hydroxyestrone, 16-ketoestrone, and 2-methoxyestrone) have been studied with respect to the influence of exciting radiation wavelength, time of heating, acid concentration, and the addition of ethanol or toluene–ethanol. These data are evaluated with respect to optimum fluorescence intensity and specificity.