THE FLUORESCENCE AND ABSORPTION SPECTRA OF OESTRONE, OESTRADIOL-17β, AND OESTRIOL COMPOUNDS
- 1 July 1953
- journal article
- research article
- Published by Bioscientifica in Journal of Endocrinology
- Vol. 9 (3) , 251-260
- https://doi.org/10.1677/joe.0.0090251
Abstract
1. Fluorescence and absorption spectra are given for oestrone, oestradiol-17β, and oestriol compounds, in response to exciting light of five different wavelengths. 2. An estimate has been made of the 'fluorescence efficiency' of each exciting wavelength. 3. On the basis of the above data, recommendations for sources of light and filters for the routine fluorimetric estimation of oestrogens are put forward.Keywords
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