SOUTHERN ANALYSIS OF GENOMIC ALTERATIONS IN GAMMA-RAY-INDUCED APRT- HAMSTER CELL MUTANTS
Open Access
- 31 May 1986
- journal article
- research article
- Published by Oxford University Press (OUP) in Genetics
- Vol. 113 (2) , 405-415
- https://doi.org/10.1093/genetics/113.2.405
Abstract
The role of genomic alterations in mutagenesis induced by ionizing radiation has been the subject of considerable speculation. By Southern blotting analysis we show here that 9 of 55 (approximately 1/6) gamma-ray-induced mutants at the adenine phosphoribosyl transferase (aprt) locus of Chinese hamster ovary (CHO) cells have a detectable genomic rearrangement. These fall into two classes: intragenic deletions and chromosomal rearrangements. In contrast, no major genomic alterations were detected among 67 spontaneous mutants, although two restriction site loss events were observed. Three gamma-ray-induced mutants were found to be intragenic deletions; all may have identical break-points. The remaining six gamma-ray-induced mutants demonstrating a genomic alteration appear to be the result of chromosomal rearrangements, possibly translocation or inversion events. None of the remaining gamma-ray-induced mutants showed any observable alteration in blotting pattern indicating a substantial role for point mutation in gamma-ray-induced mutagenesis at the aprt locus.This publication has 18 references indexed in Scilit:
- Detection of specific sequences among DNA fragments separated by gel electrophoresisPublished by Elsevier ,2006
- THE MOLECULAR GENETICS OF CELLULAR ONCOGENESAnnual Review of Genetics, 1984
- Activation of a c-K- ras Oncogene by Somatic Mutation in Mouse Lymphomas Induced by Gamma RadiationScience, 1984
- Spectra of Base Substitution Mutations Induced in Escherichia coli by Tritiated Water and the Decay of Incorporated Tritiated ThymidineRadiation Research, 1984
- Organization of a Chinese hamster ovary dihydrofolate reductase gene identified by phenotypic rescue.Molecular and Cellular Biology, 1983
- Structure of mutant alleles at the aprt locus of Chinese hamster ovary cellsJournal of Molecular Biology, 1983
- Deletion and amplification of the HGPRT locus in Chinese hamster cells.Molecular and Cellular Biology, 1983
- Alterations of Gene Structure in Ethyl Methane Sulfonate-Induced Mutants of Mammalian CellsMolecular and Cellular Biology, 1982
- γ-ray induced mutational spectrum in the lacI gene of escherichia coliMutation Research - Fundamental and Molecular Mechanisms of Mutagenesis, 1980
- Labeling deoxyribonucleic acid to high specific activity in vitro by nick translation with DNA polymerase IJournal of Molecular Biology, 1977