Alterations of Gene Structure in Ethyl Methane Sulfonate-Induced Mutants of Mammalian Cells
Open Access
- 1 November 1982
- journal article
- Published by Taylor & Francis in Molecular and Cellular Biology
- Vol. 2 (11) , 1459-1462
- https://doi.org/10.1128/mcb.2.11.1459
Abstract
To determine the types of alterations in gene structure induced by DNA-alkylating agents, we analyzed the restriction enzyme cleavage patterns of adenine phosphoribosyltransferase gene sequences in mutant strains of Chinese hamster ovary cells deficient in this enzyme. Base pair changes as detected by loss of restriction enzyme sites were found, but no major internal gene rearrangements could be detected.Keywords
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