Stimulation of Gonadotropin Release by a Non-GnRH Peptide Sequence of the GnRH Precursor
- 4 April 1986
- journal article
- research article
- Published by American Association for the Advancement of Science (AAAS) in Science
- Vol. 232 (4746) , 68-70
- https://doi.org/10.1126/science.3082009
Abstract
The human gonadotropin-releasing hormone (GnRH) precursor comprises the GnRH sequence followed by an extension of 59 amino acids. Basic amino acid residues in the carboxyl terminal extension may represent sites of processing to biologically active peptides. A synthetic peptide comprising the first 13 amino acids (H ⋅ Asp-Ala-Glu-Asn-Leu-Ile-Asp-Ser-Phe-Gln-Glu-Ile-Val ⋅ OH) of the 59-amino acid peptide was found to stimulate the release of gonadotropic hormones from human and baboon anterior pituitary cells in culture. The peptide did not affect thyrotropin or prolactin secretion. A GnRH antagonist did not inhibit gonadotropin stimulation by the peptide, and the peptide did not compete with GnRH for GnRH pituitary receptors, indicating that the action of the peptide is independent of the GnRH receptor. The GnRH precursor contains two distinct peptide sequences capable of stimulating gonadotropin release from human and baboon pituitary cells.Keywords
This publication has 17 references indexed in Scilit:
- A prolactin-inhibiting factor within the precursor for human gonadotropin-releasing hormoneNature, 1985
- Characterization of cDNA for precursor of human luteinizing hormone releasing hormoneNature, 1984
- POLYPROTEIN GENE EXPRESSION: Generation of Diversity of Neuroendocrine PeptidesAnnual Review of Biochemistry, 1984
- The human hypothalamic LHRH precursor is the same size as that in rat and mouse hypothalamusBiochemical and Biophysical Research Communications, 1983
- Synthesis and biological activity of [D-Trp6] chicken luteinizing hormone-releasing hormonePeptides, 1983
- Occurrence of higher molecular forms of LHRH in fractionated extracts from rat hypothalamus, cortex and placentaMolecular and Cellular Endocrinology, 1981
- Higher molecular weight immunoreactive species of luteinizing hormone releasing hormone: Possible precursors of the hormoneBiochemical and Biophysical Research Communications, 1977
- Production of Antiserum to LH-Releasing Hormone (LH-RH) Associated with Gonadal Atrophy in Rabbits: Development of Radioimmunoassays for LH-RHEndocrinology, 1973
- Biosynthesis of the luteinizing hormone releasing hormone in mitochondrial preparations and by a possible pantetheine-template mechanismBiochemical and Biophysical Research Communications, 1973
- Biosynthesis in,vitro of the luteinizing releasing hormone by hypothalamic tissueBiochemical and Biophysical Research Communications, 1972