The role of the imidazolyl nitrogen atoms of histidine-12 in ribonuclease S
- 1 May 1977
- journal article
- Published by Portland Press Ltd. in Biochemical Journal
- Vol. 163 (2) , 385-387
- https://doi.org/10.1042/bj1630385
Abstract
S-peptide (residues 1--14) analogues in which the active histidine-12 residue is replaced by Npi-methyl-L-histidine, Ntau-methyl-L-histidine and beta-(pyrid-3-yl)-L-alanine were synthesized and tested for their capacity to bind to S-protein and to activate it. The results show that both imidazolyl nitrogen atoms are required for optimal catalytic functioning, Ntau being essential to the catalytic reaction itself, Npi playing a role in keeping the imidazole ring in the correct position.Keywords
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