Radiation-induced Thymine Base Damage and Its Excision Repair in Active and Inactive Chromatin of HeLa Cells
- 1 January 1985
- journal article
- research article
- Published by Taylor & Francis in International Journal of Radiation Biology
- Vol. 48 (5) , 691-700
- https://doi.org/10.1080/09553008514551791
Abstract
The extent of production and excision repair of 5,6-dihydroxydihydrothymine type base (t′) damage was determined in transcriptionally active and inactive chromatin of HeLa cells after exposure to 6·8 MeV electrons. It was observed that not only the yield but also rate of repair of t′ products was greater in the active chromatin compared to the inactive chromatin of HeLa cells. The results strongly indicate that the conformation of chromatin is an important factor in determining the sensitivity to radiation damage and accessibility to enzymes required for repair of such damage.Keywords
This publication has 34 references indexed in Scilit:
- Hypersensitivity of DNA in transcriptionally active chromatin to ionizing radiationBiochimica et Biophysica Acta (BBA) - Gene Structure and Expression, 1982
- Carcinogen aflatoxin B1 is located preferentially in internucleosomal deoxyribonucleic acid following exposure in vivo in rainbow troutBiochemistry, 1980
- Chromatin Fractionation and the Properties of Transcriptionally Active Regions of ChromatinPublished by Springer Nature ,1979
- Nucleosome structure controls rates of excision repair in DNA of human cellsNature, 1977
- Selective digestion of transcriptionally active ovalbumin genes from oviduct nuclei.Proceedings of the National Academy of Sciences, 1976
- Structure of transcriptionally active chromatin.Proceedings of the National Academy of Sciences, 1975
- Biophysical Studies on the Mechanism of Quinacrine Staining of ChromosomesBiochemistry, 1974
- Partial Purification of the Template-Active Fraction of Chromatin: A Preliminary ReportProceedings of the National Academy of Sciences, 1974
- Unstable nuclear RNA synthesis following estrogen stimulationBiochimica et Biophysica Acta (BBA) - Nucleic Acids and Protein Synthesis, 1970
- A study of the conditions and mechanism of the diphenylamine reaction for the colorimetric estimation of deoxyribonucleic acidBiochemical Journal, 1956