MECHANISM OF HISTAMINE RELEASE FROM RAT MAST CELLS INDUCED BY THE IONOPHORE A23187: EFFECTS OF CALCIUM AND TEMPERATURE

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Abstract
The mechanism of histamine release from a pure population of rat mast cells induced by the lipid soluble antibiotic, A23187, has been studied and compared with data for anaphylactic histamine release reported in the literature. Histamine release induced by A23187 in the presence of calcium 10−3 mol/1 was completed in 10 minutes. By preincubation of the mast cells with A23187 for lOmin in the absence of calcium the histamine release induced by calcium, 10−3 mol/1 or 5 × 10−3 mol/1, was completed in 90s and 45 s, respectively. A23187‐induced histamine release was maximal with calcium 10−3 mol/1 when the cells were incubated at 33 to 39°C for 10 minutes. The cellular mechanism, which was stimulated by A23187 and calcium for the release of histamine, was irreversibly inactivated by incubation at 45°C. An inhibition of energy metabolism was excluded as the cause of the heat inactivation. The dependence of A23187‐induced histamine release on calcium and temperature, the time course of histamine release and the heat inactivation are consistent with the view that the same mechanism is involved in A23187‐induced and anaphylactic histamine release.