The Metabolism of Monosaccharides in Isolated Rat Mast Cells and its Influence on Histamine Release Induced by Adenosine‐5‘‐Triphosphate.

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Abstract
Histamine release from isolated rat mast cells induced by extracellularly applied ATP in the presence of calcium was enhanced by D(+)glucose and D(+)mannose, but not by D(‐)‐fructose or D(+) galactose (0.6 mM of each). Only those monosaccharides which enhanced the histamine release were metabolized by the mast cells through the Embden‐Meyerhof pathway as judged from the accumulation of lactate in samples incubated with the sugars. Lactate (1 mM) as well as pyruvate (1 mM) also enhanced the histamine release induced by ATP. Antimycin A (10‐7M) completely inhibited ATP‐induced histamine release irrespectively of the presence of glucose, pyruvate or lactate. Iodoacetate (10‐4M) blocked the enhancing effect of glucose, but not that of pyruvate or lactate. In the presence of glucose (0.6 mM) or mannose (0.6 mM) the accumulation of lactate in suspensions of rat mast cells was enhanced by antimycin A (10‐7M) and blocked by iodoacetate (10‐4M). In the absence of glycolytic substrates and metabolic inhibitors no accumulation of lactate was noted whereas in the presence of antimycin A (10‐7M) a slight but statistically significant accumulation occurred.