The Efects of Compound 48/80 and Distilled Water on the Adenosine Triphosphate Content of Isolate Rat Mast Cells

Publisher Website
Google Scholar
Abstract
The ATP content of isolated pritoneal mast cells from 15 rats averaged 1.7 ± 0.1 mmoles/kg dry weight. When degranulation of the cells and histamine release was induced by compound 48/80 no significant decrease in the ATP content could be demonstratred. When buffered distilled water was used instead as degranulating and histamine releasing agent the ATP content of the cells decreased by 65—75 per cent with an apparent t1/2 of about 20 sec. The result are descussed in relation to the postulated energy‐dependent reaction involved in the degranulation by compound 48/80.