Histamine Release from Isolated Rat Peritoneal Mast Cells Induced by Adenosine‐5‘‐Triphosphate

Abstract
Histamine release from isloated rat mast cell is induced by ATP (10‐5— 3 × 10‐5M). The activity found in an ADP‐perepatation was shown to be due to contamination with ATP, purified ADP has no activity. AMP, 3′ 5′ AMP, PCr and PEP were without histamine releasing activity. Histamine release induced by ATP was compared with that caused by compound 48/80 with respect to the influence of Ca++, Mg++, Zn++, ouabain, DNP, oligomaycin and glucose. The results are interpreted to indicate that ATP and compoundf 48/80 release histamine by mechanisms that in certain respects differ from each other. The histamine releasing effect of ATP is discussed in relation to the enery‐requiring mechanism that iis known to be involved in histamine release by compound 48/80.