A new, rapid and precise method for measuring the intra-cellular water content by gas chromatography and3H-sucrose

Abstract
A new rapid method for measuring intra-cellular water content by gas chromatography and an isotopic (3H-sucrose) technique is described. Water content of circulating red cells was revealed to be 71.26±0.31%. Intra-cellular water content increased to 102.9% of its original value following addition of 3 volumes of acid-citrate-dextrose (ACD) to 20 volumes of blood.