DNA Displacement Assay Integrated into Microfluidic Channels
- 21 May 2004
- journal article
- Published by American Chemical Society (ACS) in Analytical Chemistry
- Vol. 76 (13) , 3655-3659
- https://doi.org/10.1021/ac035238v
Abstract
This paper describes the development of a unique fluorescence-based DNA diagnostic microfluidic assay that does not require labeling of the target sequence prior to analysis. The assay is based on the displacement of a short sacrificial fluorescent-tagged indicator oligomer by a longer untagged target sequence as it is electrophoresed through a DNA-containing hydrogel plug immobilized in a microfluidic channel. The distinct advantages of this assay are the short sensing times, as a result of directed electrophoretic transport of target DNA to the sensing element, combined with the ability to detect nonlabeled target DNA.Keywords
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