Immunospecificity and quantitation of an enzyme-linked immunosorbent assay for group B streptococcal antibody

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Abstract
Type-specific antigen was purified from the supernatant of type III group B streptococcal cultures, tyrosylated and bound to microtiter wells for an enzyme-linked immunosorbent assay. The immunological specificity of the antigen and the assay was shown by reaction only with homologous unabsorbed rabbit sera and inhibition after incubation of human serum with homologous but not heterologous purified antigen. The assay was quantitated by relating optical density readings to absolute amounts of human IgG bound to the microtiter wells.