High-speed, miniaturized fluorescence microscopy in freely moving mice
Top Cited Papers
- 5 October 2008
- journal article
- research article
- Published by Springer Nature in Nature Methods
- Vol. 5 (11) , 935-938
- https://doi.org/10.1038/nmeth.1256
Abstract
A miniature epifluorescence microscope that can be carried by a freely-moving adult mouse allows cellular-level imaging of neuronal spiking or measurement of microcirculation during normal behavioral activities. A central goal in biomedicine is to explain organismic behavior in terms of causal cellular processes. However, concurrent observation of mammalian behavior and underlying cellular dynamics has been a longstanding challenge. We describe a miniaturized (1.1 g mass) epifluorescence microscope for cellular-level brain imaging in freely moving mice, and its application to imaging microcirculation and neuronal Ca2+ dynamics.This publication has 18 references indexed in Scilit:
- New Angles on Neuronal Dendrites In VivoJournal of Neurophysiology, 2007
- Imaging Large-Scale Neural Activity with Cellular Resolution in Awake, Mobile MiceNeuron, 2007
- Fiberoptic System for Recording Dendritic Calcium Signals in Layer 5 Neocortical Pyramidal Cells in Freely Moving RatsJournal of Neurophysiology, 2007
- Visualizing the Cortical Representation of Whisker Touch: Voltage-Sensitive Dye Imaging in Freely Moving MiceNeuron, 2006
- Two-Photon Imaging of Cortical Surface Microvessels Reveals a Robust Redistribution in Blood Flow after Vascular OcclusionPLoS Biology, 2006
- Fiber-optic fluorescence imagingNature Methods, 2005
- Cortical calcium waves in resting newborn miceNature Neuroscience, 2005
- In Vivo Mammalian Brain Imaging Using One- and Two-Photon Fluorescence MicroendoscopyJournal of Neurophysiology, 2004
- A Miniature Head-Mounted Two-Photon MicroscopeNeuron, 2001
- Dynamic organization of motor control within the olivocerebellar systemNature, 1995