Biology of cloned cytotoxic T lymphocytes specific for lymphocytic choriomeningitis virus: clearance of virus in vivo

Publisher Website
Google Scholar
Abstract
The data show that 1-1.5 .times. 107 lymphocytic choriomeningitis virus-specific, H-2 restricted cloned cytotoxic T lymphocytes (CTL) administered i.v. into acutely infected mice totally cleared virus from the spleens (104-105 PFU/spleen reduced to < 50 PFU/spleen) by 24 h. This activity was genetically restricted in that cloned CTL could reduce titers of infectious virus in syngeneic C57BL/6 mice but not allogeneic BALB/c mice. Dose-response analysis indicated that at least 3-5 .times. 106 cloned CTL injected i.v. were needed to reduce significant amounts of infectious virus in the spleens. No infectious virus could be recovered from the spleens for at least 4 days after injection of cloned CTL. Hence, CTL play a major role in elimination of infectious virus from spleens during lymphocytic choriomeningitis virus infection. Evidently, cloned CTL propagated in vitro for long periods of time can mediate a biologically relevant effect in vivo. These cells should be of considerable value in defining the precise manner in which CTL bring about control of viral infection, analyzing lymphocyte trafficking and the potential use of cloned CTL in immunotherapy against viral disease.

This publication has 24 references indexed in Scilit: