AXENIC TISSUE CULTURES FROM THE SPOROPHYTES OFLAMINARIA DIGITATAANDLAMINARIA HYPERBOREA(PHAEOPHYTA)

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Abstract
Starting material for the tissue cultures was the meristematic basal zone of the blade. Pieces treated 30–60 sec in hypochlorite solution were rinsed and placed on agar plates made from the artificial seawater ASP6 F2 solidified with 6 g agar l−1. After 6 weeks colorless callus‐like tissue grew out from some pieces. Treatment with activated charcoal removed some inhibiting substances from the agar medium as numbers of callus developing pieces increased on such plates. A combination of 10−5M NAA and 5 · 10−7M kinetin gave a yellow‐brown tissue. A differentiation in the tissue fromL. hyperboreawas observed as well as the formation of meiospores, which grew out into male and female plants. Thalli of sporophytes were observed but they never reached a length of more than one mm before they died or changed to an irregular pattern of growth.