Replication of Wild-Type and Mutant Human Cytomegalovirus in Life-Extended Human Diploid Fibroblasts
- 15 November 2000
- journal article
- research article
- Published by American Society for Microbiology in Journal of Virology
- Vol. 74 (22) , 10816-10818
- https://doi.org/10.1128/jvi.74.22.10816-10818.2000
Abstract
A cDNA encoding the catalytic subunit of human telomerase was used to generate life-extended derivatives of primary human diploid fibroblasts. The life-extended cells supported efficient human cytomegalovirus (HCMV) replication. A subclone of the life-extended cells was generated containing the HCMV UL82 gene and used to isolate and propagate a virus that exhibited a profound growth defect after infection at a low input multiplicity.Keywords
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