Transient gene expression in untransformed lymphocytes
- 1 January 1987
- journal article
- research article
- Published by Wiley in European Journal of Immunology
- Vol. 17 (10) , 1499-1502
- https://doi.org/10.1002/eji.1830171017
Abstract
Conditions for transfection of normal, freshly isolated and mitogen‐stimulated spleen cells were established. A sharp optimum of plasmid at 10 μg per 1.5 × 107 transfected spleen cells per transfection was observed. The Ig promoter and the IgH enhancer activity was found to be restricted to cells of the B lineage also in normal, freshly isolated lymphocytes in vitro.This publication has 11 references indexed in Scilit:
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