Enhancement by α-tocopheryl hemisuccinate of nitric oxide production induced by lypopolysaccharide and interferon-γ through the upregulation of protein kinase C in rat vascular smooth muscle cells
Open Access
- 22 April 2002
- journal article
- research article
- Published by Wiley in European Journal of Biochemistry
- Vol. 269 (9) , 2367-2372
- https://doi.org/10.1046/j.1432-1033.2002.02894.x
Abstract
The effect of α-tocopheryl hemisuccinate (TS) on lipopolysaccharide (LPS)/interferon-γ (IFN)-induced nitric oxide production in rat vascular smooth muscle cells (VSMC) was examined. The LPS/IFN-induced NO production was enhanced by TS but not by the other α-tocopherol (α-T) derivatives α-tocopheryl acetate (TA) and α-tocopheryl nicotinate (TN), or α-T itself. α-T, TA and TN inhibited the enhancement by TS of LPS/IFN-induced NO production. The enhancing effect of TS was observed in the presence of LPS, but not IFN, suggesting that TS participates in the LPS-stimulated signal pathway leading to NO production. Protein kinase C (PKC) inhibitors, but not protein kinase A inhibitors, inhibited the enhancing effect of TS on LPS/IFN-induced NO production. Furthermore, TS enhanced the amount of PKCα in VSMC. From these results, we concluded that the enhancing effect of LPS/IFN-induced NO production was caused by upregulation of PKC in VSMC.Keywords
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