A formalin fixative for immunochemical and ultrastructural studies on gastrointestinal endocrine cells.

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Abstract
Using indirect immunofluorescence, indirect immunoperoxidase and unlabeled antibody enzyme techniques, gastrin, pancreatic glucagon, insulin and somatostatin were localized in sections of human wax- and resin-embedded tissues that were fixed in a buffered formalin solution. Ultrastructural preservation of the resin-embedded samples was also adequate for combined EM and light microscope immunochemistry. As the fixative concerned is stable it can be permanently available in surgical units. This fixative may prove useful as an alternative to buffered formaldehyde, in institutions where specimen collection is difficult or which have to refer cases with an endocrine involvement to other laboratories for immunochemical and fine structural examination.

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