Complete identification of endocrine cells in the gastrointestinal tract using semithin-thin sections to identify motilin cells in human and animal intestine.
Open Access
- 1 March 1975
- Vol. 16 (3) , 225-229
- https://doi.org/10.1136/gut.16.3.225
Abstract
The present (Wiesbaden) classification of gut endocrine cells relies mainly on ultrastructure and the results of silver impregnation. Correlation with hormone production requires parallel immunology but conventional immune cytochemistry is, in most cases, difficult or at present impossible. The serial, semithin-thin-section technique offers an alternative, which provides absolute correlation between cell type and hormone production. The successful use of the technique is illustrated here by localization of motilin in the enterochromaffin cells of the small intestine.Keywords
This publication has 21 references indexed in Scilit:
- Enterochromaffin cells of the mammalian small intestine as the source of motilinVirchows Archiv B Cell Pathology, 1974
- ULTRASTRUCTURAL IMMUNOCYTOCHEMICAL LOCALIZATION OF PROLACTIN IN RAT ANTERIOR PITUITARY BY USE OF THE UNLABELED ANTIBODY ENZYME METHODJournal of Histochemistry & Cytochemistry, 1974
- ELECTRON MICROSCOPIC STUDY OF THE ADRENOCORTICOTROPIN-PRODUCING CELL WITH THE USE OF UNLABELED ANTIBODY AND THE SOLUBLE PEROXIDASE-ANTIPEROXIDASE COMPLEXJournal of Histochemistry & Cytochemistry, 1972
- Carbodiimide fixation for electron microscopy and immunoelectron cytochemistryCellular and Molecular Life Sciences, 1972
- The Further Purification of Motilin, a Gastric Motor Activity Stimulating Polypeptide from the Mucosa of the Small Intestine of HogsCanadian Journal of Physiology and Pharmacology, 1971
- Common cytochemical and ultrastructural characteristics of cells producing polypeptide hormones (the APUD series) and their relevance to thyroid and ultimobranchial C cells and calcitoninProceedings of the Royal Society of London. B. Biological Sciences, 1968
- ENZYME-LABELED ANTIBODIES FOR THE LIGHT AND ELECTRON MICROSCOPIC LOCALIZATION OF TISSUE ANTIGENSThe Journal of cell biology, 1967
- ENZYME-LABELED ANTIBODIES: PREPARATION AND APPLICATION FOR THE LOCALIZATION OF ANTIGENSJournal of Histochemistry & Cytochemistry, 1966
- 5-Hydroxytryptophan Uptake by Dog Thyroid ‘C’ Cells, and its Possible Significance in Polypeptide Hormone ProductionNature, 1966
- STUDIES ON ANTIBODY PRODUCTIONThe Journal of Experimental Medicine, 1955