Serotonin-activated adenylate cyclase and the possible role of cyclic amp in modulation of buccal muscle contraction inaplysia

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Abstract
The possible role of cyclic AMP in mediating opposite modulatory effects of serotonin (5‐HT) on Aplysia buccal mass muscles E1 and E2 was examined. Serotonin enhances E1 contractions and inhibits E2 contractions. Adenylate cyclase in membranes of both E1 and E2 is stimulated approximately 180% by 10−6M 5‐HT and 300% by 10−3M 5‐HT. Dibutyryl cyclic AMP and 8‐benzylthio cyclic AMP mimicked the effect of 5‐HT on E1 but had no effect on E2. Theophylline (Th) and isobutylmethylxanthine (IBMX) mimicked the effect of 5‐HT on E1 at high concentrations. Concentrations of Th and IBMX low enough not to have any direct effect on contraction increased both the magnitude and duration of the effect of 5‐HT on E1 contraction. Neither Th nor IBMX had a direct effect on E2 contraction, although Th produced a small increase in the effect of 5‐HT on E2. These data are consistent with the hypothesis that cyclic AMP mediates the enhancement effect of 5‐HT on E1 contraction. Other mechanisms probably mediate the effect of 5‐HT on E2 contraction.

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